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The fungal metabolite epichlorohydrin (EpiCHO) is required for the induction of microsomal drug-metabolizing enzymes (xenobiotic epoxide-forming enzymes). The nature of the xenobiotic-metabolizing activities induced by a variety of dietary and environmental chemicals has been studied in hepatic and intestinal microsomes from various strains of rodents and other mammals. Microsomes of EpiCHO-induced animals exhibit a characteristic induction of microsomal glutathione-S-transferase activity and higher levels of total cytochrome P450. Hepatic microsomes isolated from 6-week-old EpiCHO-induced rats are approximately fivefold more active than microsomes from untreated rats in the dealkylation of 1-chloro-2,4-dinitrobenzene to form an epoxide product (oxiran). In contrast, microsomes of untreated rats are about 10-fold more active than microsomes of treated animals in the dealkylation of 1,2-dichlorobenzene to form the 1,2-dichloroaniline, 1-chloro-4-nitrobenzene, or the 2-nitro-1-chloroaniline product. The induction of aryl hydrocarbon hydroxylase (AHH) activity in the livers of adult rats is correlated with the activity of a major xenobiotic epoxide-forming enzyme, microsomal glutathione-S-transferase. Hepatic microsomes from untreated rats exhibit approximately 5-fold more AHH activity than microsomes from EpiCHO-induced rats. Hepatic and intestinal microsomes from untreated and EpiCHO-induced rats exhibit similar activities in the epoxidation of polycyclic aromatic hydrocarbons. These data indicate that the EpiCHO metabolite is a critical agent that induces the xenobiotic epoxide-forming enzymes. 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System Requirements For Cpukiller3: Recommended: Minimum: Tutorials: Written Tutorial: Screenshots: Inspector Gadget | 01.05 | 2011 | HARDWARE, MENU, COMPRESSED | 136.8 MB Inspector Gadget | 01.07 | 2011 | HARDWARE, MENU, COMPRESSED | 133.9 MB Inspector Gadget | 01.10 | 2011 | HARDWARE, MENU, COMPRESSED | 137.3 MB
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